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qki7  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank qki7
    Inducible QKI5, QKI6, and <t>QKI7</t> expression cells are established in H1-8 lines (H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind ). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8- QKI5 ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8- QKI6 ind and H1-8- QKI7 ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.
    Qki7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qki7/product/Developmental Studies Hybridoma Bank
    Average 90 stars, based on 1 article reviews
    qki7 - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function"

    Article Title: QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function

    Journal: Nature Communications

    doi: 10.1038/s41467-020-20327-5

    Inducible QKI5, QKI6, and QKI7 expression cells are established in H1-8 lines (H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind ). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8- QKI5 ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8- QKI6 ind and H1-8- QKI7 ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.
    Figure Legend Snippet: Inducible QKI5, QKI6, and QKI7 expression cells are established in H1-8 lines (H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind ). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8- QKI5 ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8- QKI6 ind and H1-8- QKI7 ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.

    Techniques Used: Expressing, Immunofluorescence, Staining



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    Image Search Results


    Inducible QKI5, QKI6, and QKI7 expression cells are established in H1-8 lines (H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind ). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8- QKI5 ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8- QKI6 ind and H1-8- QKI7 ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.

    Journal: Nature Communications

    Article Title: QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function

    doi: 10.1038/s41467-020-20327-5

    Figure Lengend Snippet: Inducible QKI5, QKI6, and QKI7 expression cells are established in H1-8 lines (H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind ). Representative images of immunofluorescence staining of TNNT2 and ACTN2 in H1-8- QKI5 ind , H1-8- QKI6 ind , H1-8- QKI7 ind cardiomyocytes with or without doxycycline induction. Myofibrillar structures are recovered in doxycycline-induced H1-8- QKI5 ind cardiomyocytes (Day-15) and ACTN2 expression is also recovered. However, doxycycline-induced H1-8- QKI6 ind and H1-8- QKI7 ind cardiomyocytes fail to recover ACTN2 expression and generate normal myofibrillar structures (Day-15). Scale bar: 25 μm. All experiments are independently repeated three times with three different sets of cell samples to confirm the reproducibility of the findings.

    Article Snippet: The membranes were blocked with 5% nonfat dry milk (Bio-Rad, 1706404) in Tris-buffered saline containing Tween-20 and incubated with primary antibodies against TNNT2 (DSHB, Cat#AB528495), ACTN2 (Sigma, Cat#A7811), MF20 (DSHB, Cat#AB2147781), MYOZ2 (ThermoFisher, Cat#PA5-76946), TNNI3 (Santa Cruz, Cat#SC15368), Tubulin (Sigma, Cat#T6199), Pan-QKI(Abcam, Cat#ab126742), QKI-5 (Bethyl, A300-183A), QKI6 (Millipore, Cat#AB9906), and QKI7 (Millipore, Cat#AB9908), respectively, at 1 : 1000 dilution, and corresponding secondary antibodies labeled with horseradish peroxidase (Goat anti-Mouse IgG antibody, ThermoFisher Scientific, G21040; Mouse anti-Rabbit IgG antibody: Santa Cruz, sc-2357) at 1 : 4000 dilution.

    Techniques: Expressing, Immunofluorescence, Staining